Modulating graft vs host response by T and Natural Killer cells photoimmunotherapy controlled by two-photons absorbing switches
Chemists : A. Pasc and M. Mourer in L2CM,
Physicists : M. Tanaka, Heidelberg Univ.,
Biologists : V. Décot-IMoPa.
The immuno-PAS project aims to design and demonstrate the immunomodulatory properties of new ON/OFF photoresponsive molecular tools on T and NK cells, through serine/Tim-3 binding, targeting thus applications in GVHD and autoimmune diseases. To this aim, the effects of swPS will be evaluated on T and NK cells isolated from GVHD patients. The originality of this approach consists in using two-photon absorbing photoswitches that should allow a better spatial and temporal selectivity compared to UV-Vis chromophores reported to date, thanks to the irradiation at a focal point rather than in a plane. The decreased photobleaching, and therefore photodegradation, is another notable advantage of two-photon absorption, that should allow more precise phenotype analysis of cells.
